RAN Antibody MA1-20581 from Thermo Fisher Scientific

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Western blot was performed using Anti-RAN Monoclonal Antibody (Product # MA1-20581) and a 28 kDa band corresponding to RAN was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of A549 (Lane 1), HeLa (Lane 2), NIH/3T3 (Lane 3), Jurkat (Lane 4), MDA-MB-231 (Lane 5), MCF7 (Lane 6), NK-92 (Lane 7), tissue extracts of Mouse Brain (Lane 8), Mouse Testis (Lane 9) and Rat Testis (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
ICC/IF

Immunofluorescence analysis of RAN was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAN Monoclonal Antibody (ARAN1) (Product # MA1-20581) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing staining in nucleus and cytoplasm. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
RAN Antibody

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Thermo Fisher Scientific for
RAN Antibody

The RAN Antibody from Thermo Fisher Scientific is a Mouse Monoclonal antibody to Ran, RAN, member RAS oncogene family L homeolog, and ran.L. This antibody recognizes Homo Sapiens, Human, Mouse, Rat, Bovine, Hamster, and Xenopus/Amphibian antigen. The RAN Antibody has been validated for the following applications: EIA, Immunoassay, ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blot.

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RAN Antibody

RAN Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

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RAN Antibody from Thermo Fisher Scientific

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